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yfnI [2018-12-07 15:41:01]
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yfnI [2018-12-07 15:41:01]

polyglycerolphosphate lipoteichoic acid synthase, general stress protein, major secreted protein, required for survival at low temperature (4°C)
Locus
BSU07260
Isoelectric point
5.95
Molecular weight
73.14 kDa
Protein length
639 aa Sequence Blast
Gene length
1917 bp Sequence Blast
Function
biosynthesis of lipoteichoic acid
Product
minor lipoteichoic acid synthetase
Essential
no
Synonyms
yetP

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
796,314 → 798,233

Phenotypes of a mutant

  • induction of SigM and SigX activities PubMed
  • more sensitive to nisin PubMed
  • increased conjugation of ICEBs1 PubMed
  • suppression of the growth and morphology defects of a glmR mutant PubMed
  • The protein

    Protein family

  • LTA synthase family (according to Swiss-Prot)
  • Paralogous protein(s)

    Modification

  • phosphorylation on (Thr-311 OR Ser-312) PubMed
  • can be phosphorylated by PrkC in vitro PubMed
  • Structure

  • 2W5Q (LtaS from S. aureus, corresponds to aa 215 ... 632 of YfnI) PubMed
  • Localization

  • extracellular (signal peptide) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of yfnI PubMed
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    Biological materials

    Mutant

  • MGNA-C226 (yfnI::erm), available at the NBRP B. subtilis, Japan
  • SM-NB (yfnI-spc), available in Anne Galinier's and Boris Görke's labs
  • GP1390 yfnI::spc, available in Jörg Stülke's lab
  • GP1397 yfnI::ermC, available in Jörg Stülke's lab
  • BKE07260 (ΔyfnI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCAACCTCTACTTTCT, downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
  • BKK07260 (ΔyfnI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGGCAACCTCTACTTTCT, downstream forward: _UP4_TAAGATGAAAAAGAGCCTTG
  • References

    Reviews

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    Original publications

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